[Histonet] decal protocol with EDTA for bonemarrow biopsies

[Gudrun Lang]

Dear histonetters!

I would be happy about some input about decalcification protocols with EDTA
of trephine bonemarrow biopsies.

recommended duration of fixation?

recommended duration of decalcification?

strategies for speed-up of decal?

recommended EDTA-solution formula?

 

Hopefully some experienced histotechs can share their knowledge with me.

thanks in advance

Gudrun