[Histonet] Cut FFPE Slides for ISH

[Dorothy Hu]

Hi Jennifer,

My ISH slides (FFPE) were stored in -20oC for several months or more than
one year and the targets were less abundant. I didn't use RNAscope though,
which is more sensitive. I don't know what kind of RNA your researcher try
to locate and need to do ISH within 24 hours after cutting?  Fresh cut
section will yield more signal for sure. I think as long as you have a few
copies of genes in your tissue, RNAscope should be able to detect.

Dorothy Hu
HSDM







> Message: 2
> Date: Mon, 28 Aug 2017 15:30:08 +0000
> From: "Jennifer  Phinney" <jhill at vet.k-state.edu>
> To: "Histonet at lists.utsouthwestern.edu"
>         <Histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Cut FFPE Slides for ISH
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>
> Hello Histonet,
> I am hoping someone can point me in the right direction to find some
> references for how long FFPE slides intended for ISH can be kept before
> staining.  I have a researcher who swears their slides have to be stained
> within 24 hours of being cut, which does not sound correct to me.  I've
> used ACD's RNAscope before and their protocol lists the timeframe as 3
> months (so this is a bit of a difference).
>
> There's a lot of information about fixation times, but I've come up empty
> handed for cut slides.
>
> Thanks everyone!
>
> Jennifer Phinney QIHCCM
> Project Coordinator
> Kansas State University
> Veterinary Diagnostic Lab
>
>
>