[Histonet] Digital Pathology & Coverslipping

[Gudrun Lang]

Hi!
We scan our daily HEs for 1,5 year now. We have a Leica glass-coverslipper.
The brilliance of the images is very good. With film we had the experience,
that the brilliance of digital photos were never of the same quality as with
glass. (and had always issues with coming off the film after a year of
storage. So I doubt, that scanning older slides is successful.)
The drawback of the glass coverslipper is, that you have always to adjust
it. One day it is perfect without any bubbles, the other day the medium has
changed the consistency and you need more or less of it. When the medium
squeezes out a little bit, the scanner may recognise it as tissue. The same
happens, when the medium is spared at the edges and air goes in.
As solvent we use butyl-acetate, that evaporates very quickly and clean. The
slides are dry within minutes.
Our workflow is first to organise the cases for delivering, then scan the
sorted cases and then deliver the slides to the pathologists. So the slides
have enough time to dry and we can check the quality of coverslipping and
quality of the slide-barcode.

bye
Gudrun

-----Ursprüngliche Nachricht-----
Von: Haley Huggins via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Montag, 14. August 2017 17:48
An: Alexis Templeton
Cc: histonet at lists.utsouthwestern.edu
Betreff: Re: [Histonet] Digital Pathology & Coverslipping

Glass or film coverslips are fine, but you have to make sure they are clean,
no excess mounting media, or bubbles. The scanners pick up a lot of extra
things you don't want scanned. Also, check with your pathologists to see if
they have an opinion one way or another about which coverslips they prefer.

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*
*Cell: 303-652-7453*

On Fri, Aug 11, 2017 at 12:42 PM, Alexis Templeton via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi All!
>
> My lab is considering moving up in the world of technology.  The goal 
> is to start scanning slides for pathologists to read digitally.  We 
> are a relatively high throughput lab and I'm trying to figure out what 
> we need in terms of an automatic coverslipper to avoid drying time.  
> We currently still coverslip by hand and I'm assuming there would be 
> too much wet glue to place the slides directly in a scanner.  Tips and 
> recommendations, please!
>
> Alexis Templeton, HT (ASCP)CM
> Diagnostic Laboratory Supervisor
> Histopathology
> Texas A&M Veterinary Medical Diagnostic Laboratory P.O. Drawer 3040 | 
> College Station, TX 77841-3040
> p: (979) 845-3414 | f: (979) 845-1794
> atempleton at tvmdl.tamu.edu<mailto:atempleton at tvmdl.tamu.edu>
> http://tvmdl.tamu.edu<http://tvmdl.tamu.edu/>
>
> We Moved!  Effective February 27, 2017 our physical (shipping) address 
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