[Histonet] Nuclei isolation from ethanol-fixed tissue

[R P]

Dear Histonet Users,

 

What methods can be used for quantitative isolation of intact nuclei from
brain tissue cryosections fixed in ethanol?  The purpose is to extract RNA
from single nuclei.  The greater the yield of nuclei from small sections,
and the lower the bias against a particular cell type, the better.

 

Thank you,

 

RB

Swedish Medical Center