[Histonet] Cytology pap staining

[Tony Henwood (SCHN)]

I would also check the fixation.
Do the smears look air-dried. The larger nuclei, following air-drying do not concentrate the Hx as much as prompt alcohol fixation resulting in paler stained nuclei. 

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, 20 April 2017 2:31 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cytology pap staining

I have been put in charge of figuring out why our pap stains are light on the hematoxylin. Everything was filtered and used fresh as per usual using the same protocol we have used for the past two years.

If anyone has any suggestions as to how to fix this problem I would greatly appreciate it. If you need more information please let me know and I will get it for you to help assist you in assisting me

-- 

Charles Riley HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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