[Histonet] IF vs IHC another reason

[Terri Braud]

Another reason that IHC is used instead of IF is with IHC, one preserves the ability to see tissue/cell morphology through Light Microscopy at the same time as the visual IHC label.  Morphology is difficult to see with IF, with the exception of the fluorescein labeled area.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
-----Original Message-----
From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, 13 April 2017 11:10 PM
Hello!
I just need a help with a simple question...Is anyone can explain me what is the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks  :)
Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: Blanca.Lopez at utsouthwestern.edu