[Histonet] Questions concerning Leica Bond RX
Cathy M. Mathis/Comparative Medicine
cmmathis at wakehealth.edu
Wed Sep 28 16:04:03 CDT 2016
Hello Histo-netters!
I have a Bond RX and before I try to recreate the wheel, I thought I would ask some experts. Has anyone used a goat primary on this instrument and if so how? Also, has anyone had success with TUNEL on this instrument and if so, could you share your knowledge?
Thank you all for your time,
Cathy
Cathy M. Mathis
Laboratory Technician IV
Department of Pathology - Section on Comparative Medicine
Clarkson Campus
Medical Center Boulevard \ Winston-Salem, NC 27157
p 336.716.1538
cmmathis at wakehealth.edu
-----Original Message-----
From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu]
Sent: Wednesday, September 28, 2016 12:33 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 154, Issue 23
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Today's Topics:
1. Re: Water for H&E Stainers? (Hannen, Valerie)
2. Position announcement (Leslie, Mary)
3. Sandersons Rapid Bone stain chemistry and history (Gayle Callis)
4. Re: water for stainers (Terri Braud)
5. H.pylori question (Gareth Davis)
6. buffered zn-formalin recipe needed (Preiszner, Johanna)
7. Re: GMS (Caroline Miller)
8. Re: H.pylori question (Kienitz, Kari)
9. ATRX Antibody (Marcum, Pamela A)
10. Re: Water for H&E Stainers? (cfitz)
11. Re: Water for H&E Stainers? (WILLIAM DESALVO)
12. Re: Histonet Digest, Vol 154, Issue 22 (Nasir Abbas)
13. HT grossing competencies (Farah Nawaz)
14. looking for a cryostat service tech. in the Houston area.
(Hebert, Pamela)
15. EXAKT band saw blade (Garcia, Lori, M.Sc.)
----------------------------------------------------------------------
Message: 1
Date: Tue, 27 Sep 2016 13:10:05 -0400
From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
To: 'P Sicurello' <patpxs at gmail.com>
Cc: "Histonet at lists.utsouthwestern.edu"
<Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Water for H&E Stainers?
Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E63B at isexstore03>
Content-Type: text/plain; charset="us-ascii"
Ours is hooked up to DI water.
Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com
www.parrishmed.com
-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, September 27, 2016 12:28 PM
To: HistoNet
Subject: [Histonet] Water for H&E Stainers?
Good Morning Everyone,
What type of water do you use with your automated H&E stainers? House or deionized/distilled?
We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance.
Sincerely,
Paula
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.
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Message: 2
Date: Tue, 27 Sep 2016 17:13:54 +0000
From: "Leslie, Mary" <Mary.Leslie at tricore.org>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Position announcement
Message-ID:
<E0D95E77CCA6D74C9618B5DDC3458C98E4867EB3 at TRL-MAIL-WW.tricore.org>
Content-Type: text/plain; charset="us-ascii"
Histotechnologist needed M-F 1900 -0330
Tricore Reference Laboratories
Albuquerque, NM
Contact: laura.enriquez at tricore.org<mailto:laura.enriquez at tricore.org>
505-938-8915
------------------------------
Message: 3
Date: Tue, 27 Sep 2016 11:19:17 -0600
From: "Gayle Callis" <gayle.callis at bresnan.net>
To: "Histonet" <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Sandersons Rapid Bone stain chemistry and history
Message-ID: <004a01d218e3$47811390$d6833ab0$@bresnan.net>
Content-Type: text/plain; charset="iso-8859-1"
You worte: RBS is the same as Methylene Blue, I believe.
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:
-----Original Message-----
From: Alicia Marie Ortega [mailto:alicia.ortega at colorado.edu <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> ]
Sent: Thursday, September 22, 2016 4:50 PM
To: histonet at lists.utsouthwestern.edu <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
Subject: [Histonet] Question on Sanderson Rapid Bone Stain
Hello everyone,
I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain.
My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain. I was wondering if anyone knows of a way to intensify the staining of the RBS? I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration).
Thank you in advance for your time and help.
Sincerely,
Alicia Ortega
Postdoctoral Research Associate
University of Colorado, Boulder
***********************************************
Sandersons Rapid Bone Stain is oxidized methylene blue. It is actually a modified Stevenels blue, a polychromatic stain made by oxidizing methylene blue with potassium permanganate that produces the byproducts i.e. methylene violet, azure A, azure B, thionin, toluidine blue, thionin and possibly some other thiazine dyes .Stevenels blue was first used by Maniatopoulos et al to stain PMMA embedded, un-etched bone sections using a 60?C water bath and
optional counterstaining with either Van Gieson or basic fuchsin. Cathy
Sanderson (Mayton) found a better, easier way to make up Stevenels blue for
large production. We found it tends to be a weaker stain unless you etch
the bone with 0.5% formic acid using a sonicator for 1 minute, rinse with hot tap water very briefly, blot and look at the surface stained bone.
Over aggressive water rinsing allows the stain to release from this mildly acid etched calcified bone. All the etching is doing is a gentle removal of calcium from only a few micrometers of bone surface and allow the stain to
penetrate better. Acid etching intensified RBS staining but there are
drawbacks but ways to deal with that. You can stain etched bone sections,
view, photograph and then do a very brief (only a few quick dips), blot after counterstain, view and photograph the now counterstained section.
Avoiding over rinsing keeps the RBS in etched bone section. If things
don?t look right, merely polish the stain from surface and start over.
You can counterstain Sanderson RBS stained (etched) bone section with the two mentioned counterstains, but it has to be done very briefly or the counterstain will differentiate RBS from the bone and be overly red.
RBS, as used according to Sanderson and product brochure instructions, is for un-etched mineralize bone sections in PMMA or even EXAKT preparations.
We preferred to acid etch, do the original Stevenels Blue instead of RBS, and get a much deeper stain on osteoid, and other bone components.
Counterstaining was not done as it masked acid etched bone components excessively or removed them,
The joy of using RBS is avoiding a long, tedious, and messy making up of
Stevenels blue. One can also try to stain longer in RBS per brochure
instructions to see if that deepens the staining. Old bone picks up the
stain differently than young bone in terms of age of the animal. Different species can stain differently too.
It is important to know that repeated heating of RBS (or Stevenels) allows the portassium permanganate to continue oxidizing the methylene blue, and also keeps raising the pH to be more alkaline. One should replenish or top off the stain, always filter it into a clean coplin jar before using or reusing this stain. Eventually the stain does not work very well and you need to start with new stock.
We preferred to use a MacNeals tetrachrome combined with toluidine blue per Sterchi method for more brilliant staining of osteoid other bone components
including cartilage.
Take care
Gayle Callis
HTL/HT/MT(ASCP)
------------------------------
Message: 4
Date: Tue, 27 Sep 2016 17:36:25 +0000
From: "Terri Braud" <tbraud at holyredeemer.com>
To: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] water for stainers
Message-ID:
<48E053DDF6CE074DB6A7414BA05403F808DA66 at HRHEX02-HOS.holyredeemer.local>
Content-Type: text/plain; charset="us-ascii"
Our Prisma is hooked up to tap water.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
3. Water for H&E Stainers? (P Sicurello)
4. Re: Water for H&E Stainers? (Elizabeth Chlipala)
Message: 3
Date: Tue, 27 Sep 2016 09:28:25 -0700
From: P Sicurello <patpxs at gmail.com>
Good Morning Everyone,
What type of water do you use with your automated H&E stainers? House or deionized/distilled?
We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. Thank you in advance.
Sincerely, Paula
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
------------------------------
Message: 5
Date: Tue, 27 Sep 2016 10:45:25 -0700
From: Gareth Davis <garethdavisyuma at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] H.pylori question
Message-ID:
<CAHx9TZPjeVZH_Pyrw2Hi9faRWJQ12OG8TD5zhB_ULhebj6=zBw at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Okay, so I know I have heard rumors about the H.pylori testing being affected by Medicare. We currently do the H. pylori IHC stain on all gastric biopsies. Has anyone heard that Medicare will change, and we will no longer be able to do it on all cases? The last lab I worked in, in Tennessee, stopped doing it all cases, because of the Medicare changes.
But, here in Arizona, they are still doing it.
What's the scoop?
--
Ms. Gareth B. Davis, HT, QIHC (ASCPcm)
Yuma Gastroenterology
Yuma, AZ 85364
928-248-5259
------------------------------
Message: 6
Date: Tue, 27 Sep 2016 18:21:12 +0000
From: "Preiszner, Johanna" <PREISZNE at mail.etsu.edu>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] buffered zn-formalin recipe needed
Message-ID:
<MWHPR07MB3134BF1F305A0C29CFD682639CCC0 at MWHPR07MB3134.namprd07.prod.outlook.com>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
For the life of me I can't locate a recipe for buffered zn-formalin, nor online or off.
Could someone please help us out?
Thanks,
Hanna Preiszner
ETSU/QCOM
------------------------------
Message: 7
Date: Tue, 27 Sep 2016 11:23:47 -0700
From: Caroline Miller <mills at 3scan.com>
To: "Martin, Gary" <gmartin at marshallmedical.org>
Cc: "Histonet at Lists. Edu" <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] GMS
Message-ID:
<CAFTHRQP3meq-2yo6CxKHEwXeW5vekciK7gQ0-jTV21rZtE19zQ at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Maybe a fixation issue? Is this happening on all your slides, or just one
set.
Also, do you have a little acetic acid in the light green, I find that
helps. I also find it washes out quickly and a fast dehydration is
necessary.
Very funny that you are having issues with the LG and not the silver, lol,
histology can be so 'interesting' sometimes!
mills
On Mon, Sep 26, 2016 at 4:30 PM, Martin, Gary via Histonet <
histonet at lists.utsouthwestern.edu> wrote:
> I am having difficulties with a stain I've done many times ...GMS. The
> light green is only staining the outer edges of the specimen. Any Ideas?
>
>
> Thanks
>
> Gary
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
------------------------------
Message: 8
Date: Tue, 27 Sep 2016 19:06:22 +0000
From: "Kienitz, Kari" <kkienitz at orclinic.com>
To: Gareth Davis <garethdavisyuma at gmail.com>,
"histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] H.pylori question
Message-ID:
<CY1PR10MB0716D6EE18BFEEDC68DF863AAFCC0 at CY1PR10MB0716.namprd10.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
Local Coverage Determinations (LCD) from the website for the Centers for Medicare & Medicaid Services (CMS). Please use the link below to access this policy.
you may find this information useful. Look at the GI section. It specifically calls out the use of special stains for H.pylori. Reimbursement may be an issue for you.
L35693 MolDX: Special Histochemical Stains and Immunohistochemical Stains Palmetto GBA(11302) http://www.cms.gov/medicare-coverage-database/details/lcd-details.aspx?LCDId=35693&ver=6&ContrId=229&ContrVer=1 N
Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
1111 NE 99th Ave
Portland, OR 97220
503.935.8311
kkienitz at orclinic.com
CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you
________________________________________
From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Tuesday, September 27, 2016 10:45 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] H.pylori question
Okay, so I know I have heard rumors about the H.pylori testing being
affected by Medicare. We currently do the H. pylori IHC stain on all
gastric biopsies. Has anyone heard that Medicare will change, and we will
no longer be able to do it on all cases? The last lab I worked in, in
Tennessee, stopped doing it all cases, because of the Medicare changes.
But, here in Arizona, they are still doing it.
What's the scoop?
--
Ms. Gareth B. Davis, HT, QIHC (ASCPcm)
Yuma Gastroenterology
Yuma, AZ 85364
928-248-5259
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Tue, 27 Sep 2016 19:55:03 +0000
From: "Marcum, Pamela A" <PAMarcum at uams.edu>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] ATRX Antibody
Message-ID: <8856c26e8b844c50adb842ca38fd7958 at MAIL13M2N1.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"
I am asking this question for our IHC Supervisor. She is looking for a source for the ATRX antibody as either an RUO or preferably IVD. They use the Ventana Ultras and Benchmarks so if anyone has a procedure that would be great.
Pam
----------------------------------------------------------------------
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------------------------------
Message: 10
Date: Tue, 27 Sep 2016 14:17:08 -0700
From: cfitz <cfitz at 007group.com>
To: Elizabeth Chlipala <liz at premierlab.com>, P Sicurello
<patpxs at gmail.com>, "'histonet at lists.utsouthwestern.edu'
(histonet at lists.utsouthwestern.edu)"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Water for H&E Stainers?
Message-ID: <yn8vcl1ski3k31ssywl67qd0.1475011028116 at email.android.com>
Content-Type: text/plain; charset=utf-8
Our Prisma is connected to tap water with a prefilter that we change weekly.?
CathyBritish Columbia?
Sent from my Samsung Galaxy smartphone.
-------- Original message --------From: Elizabeth Chlipala via Histonet <histonet at lists.utsouthwestern.edu> Date: 2016-09-27 9:54 AM (GMT-08:00) To: P Sicurello <patpxs at gmail.com>, "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" <histonet at lists.utsouthwestern.edu> Subject: Re: [Histonet] Water for H&E Stainers?
Paula
We have ours hooked up to tap water.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, September 27, 2016 10:28 AM
To: HistoNet
Subject: [Histonet] Water for H&E Stainers?
Good Morning Everyone,
What type of water do you use with your automated H&E stainers?? House or deionized/distilled?
We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance.
Sincerely,
Paula
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Tue, 27 Sep 2016 16:01:08 -0700
From: WILLIAM DESALVO <wdesalvo.cac at outlook.com>
To: cfitz <cfitz at 007group.com>, Liz Chlipala <liz at premierlab.com>, P
Sicurello <patpxs at gmail.com>, histonet
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Water for H&E Stainers?
Message-ID: <COL129-W432DEBEBCB390844F785C482CC0 at phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Tap water is most often used in H&E staining for rinsing and sometimes blueing and can be very cost effective. I do suggest you use a filter prior to the water entering the instrument. The filter can eleviate one of the key concerns, contaminates.
The typical contaminates/compounds can be:
Inorganic ions: chlorides, nitrates, sulfates, sodium, calcium, iron
Organic molecules: humic acids, phenols, tannins, pesticide residues
Particles and colloids
Microorganisms and their by-products
Dissolved gases
The other key concern is that the pH of the tap water may vary by location and even season. To change the color of hematoxylin stained nuclei from redish to blue, the pH should be 7 or higher. Most tap water is typically slightly acidic (pH of around 6.0 to 6.8), but this is much more alkaline than the pH of the haematoxylin, around pH 2.7. Rinsing for two to five minutes in running tap water will remove most of the excess mordant giving sharp blue nuclear staining. If you want sharper and better defined
As my good friend Skip Brown states, a good H&E stain is "balance of coloration". Understand the chemistry and you control the balance.
William DeSalvo, BS HTL(ASCP)
> Date: Tue, 27 Sep 2016 14:17:08 -0700
> To: liz at premierlab.com; patpxs at gmail.com; histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Water for H&E Stainers?
> From: histonet at lists.utsouthwestern.edu
>
> Our Prisma is connected to tap water with a prefilter that we change weekly.
> CathyBritish Columbia
>
>
> Sent from my Samsung Galaxy smartphone.
> -------- Original message --------From: Elizabeth Chlipala via Histonet <histonet at lists.utsouthwestern.edu> Date: 2016-09-27 9:54 AM (GMT-08:00) To: P Sicurello <patpxs at gmail.com>, "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" <histonet at lists.utsouthwestern.edu> Subject: Re: [Histonet] Water for H&E Stainers?
> Paula
>
> We have ours hooked up to tap water.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> liz at premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> -----Original Message-----
> From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Tuesday, September 27, 2016 10:28 AM
> To: HistoNet
> Subject: [Histonet] Water for H&E Stainers?
>
> Good Morning Everyone,
>
>
>
> What type of water do you use with your automated H&E stainers? House or deionized/distilled?
>
>
>
> We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance.
>
> Sincerely,
>
>
>
> Paula
>
>
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Wed, 28 Sep 2016 08:39:40 +0000 (UTC)
From: Nasir Abbas <shinwari_1 at yahoo.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Histonet Digest, Vol 154, Issue 22
Message-ID: <1743394579.715511.1475051980422 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
Dear Histoneters!Good DayMay I request you guys to share protocol of frozen tissues (mouse liver) for immunoflorescence assay. Lots of protocol are available online but i will highly appreciate the one that could lead me from tissue preparation towards staining procedure as I am really blank about frozen tissues and slides dealing. So I will be on standby for your response.?Thanks in advance.
Sincerely?Nasir Abbas
---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue?Science Park?Guangzhou, 510530,?Guangdong?P.R.ChinaCell: +86-1312 8296 614
On Wednesday, 28 September 2016, 1:12, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:
Send Histonet mailing list submissions to
??? histonet at lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
??? histonet-request at lists.utsouthwestern.edu
You can reach the person managing the list at
??? histonet-owner at lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
? 1. GMS (Martin, Gary)
? 2. Re: Unprocessing tissue (Caroline Miller)
? 3. Water for H&E Stainers? (P Sicurello)
? 4. Re: Water for H&E Stainers? (Elizabeth Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Mon, 26 Sep 2016 16:30:49 -0700
From: "Martin, Gary" <gmartin at marshallmedical.org>
To: <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] GMS
Message-ID:
??? <6ED9D4252F278841A0593D3D788AF24C366E5E51 at mailsvr.MARSHMED.local>
Content-Type: text/plain;??? charset="us-ascii"
I am having difficulties with a stain I've done many times ...GMS. The
light green is only staining the outer edges of the specimen. Any Ideas?
Thanks
Gary
------------------------------
Message: 2
Date: Mon, 26 Sep 2016 16:49:14 -0700
From: Caroline Miller <mills at 3scan.com>
To: "Armstrong, Karoleigh T" <Karoleigh.Armstrong at armc.net>
Cc: "histonet at lists.utsouthwestern.edu"
??? <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Unprocessing tissue
Message-ID:
??? <CAFTHRQMn5=hqZoYF-FrJ-mF2agzEUP_gzwyjxNhGUx90hMkskA at mail.gmail.com>
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Not crazy at all, this is the kind of thing I was thinking someone would
have out there :)
I have used downey on hard tissue block faces before now too!
thanks so much for all your advice histonet :)
mills
On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet <
histonet at lists.utsouthwestern.edu> wrote:
> Hi Caroline,
>
> This may sound a little crazy,
>
> but I was working on some mummified tissue back in the 80's, (Mommy is
> old), and to deal with the dried out tissue we would give them,
>
> 1. A quick .5 percent Sodium Hydroxide soak, 5minutes
>
> 2. Wash is running water, 1 minute
>
> 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes
>
> 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes.
>
> 5. Wash with running water 2-3 minutes.
>
> Proscess your tissue as you usually would.
>
> They came out pretty darn good.
>
> KK Armstrong H.T. (ASCP)
>
>
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--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
------------------------------
Message: 3
Date: Tue, 27 Sep 2016 09:28:25 -0700
From: P Sicurello <patpxs at gmail.com>
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Water for H&E Stainers?
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Good Morning Everyone,
What type of water do you use with your automated H&E stainers?? House or
deionized/distilled?
We cannot buy one of the waterless, fancy schmancy H&E stainers at this
time.? L
Thank you in advance.
Sincerely,
Paula
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.? Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.? If you received this e-mail in error,
please contact the sender and delete the material from any computer.
------------------------------
Message: 4
Date: Tue, 27 Sep 2016 10:54:18 -0600
From: Elizabeth Chlipala <liz at premierlab.com>
To: P Sicurello <patpxs at gmail.com>,
??? "'histonet at lists.utsouthwestern.edu'
??? (histonet at lists.utsouthwestern.edu)"
??? <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Water for H&E Stainers?
Message-ID:
??? <14E2C6176416974295479C64A11CB9AE02BEEABA5B09 at SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Paula
We have ours hooked up to tap water.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, September 27, 2016 10:28 AM
To: HistoNet
Subject: [Histonet] Water for H&E Stainers?
Good Morning Everyone,
What type of water do you use with your automated H&E stainers?? House or deionized/distilled?
We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance.
Sincerely,
Paula
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer.
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End of Histonet Digest, Vol 154, Issue 22
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------------------------------
Message: 13
Date: Wed, 28 Sep 2016 14:02:38 +0000
From: Farah Nawaz <fnawaz at uhcc.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] HT grossing competencies
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<BN6PR18MB147582550AD8166C8AC54F87ADCF0 at BN6PR18MB1475.namprd18.prod.outlook.com>
Content-Type: text/plain; charset="iso-8859-1"
Hello:
I am a new AP supervisor and I am looking to add grossing duties to our HTs. They are currently being trained here in our lab.
Can someone please share an example of non-pathologist grossing annual competency checklist with me?
I just need a reference to create our own competency checklist for our HTs that gross.
Thank you,
Farah Nawaz, CT(ASCP), MA-Mgmt
Anatomic Pathology Supervisor
Union Hospital of Cecil County
Office: 410-392-7084
Fax: 443-674-1596
fnawaz at uhcc.com
CONFIDENTIALITY STATEMENT. This email and any attachment is for the sole use of the intended recipient and may contain private, confidential and/or privileged information that may be subject to Union Hospital of Cecil County internal policies. If you are not the intended recipient, any dissemination, distribution or copying is strictly prohibited. If you have received this transmission in error, please notify Union Hospital of Cecil County immediately by return email or by email to Administrator at uhcc.com and delete the message and all copies, printouts and attachments from your system.
------------------------------
Message: 14
Date: Wed, 28 Sep 2016 14:40:01 +0000
From: "Hebert, Pamela" <phebert at shrinenet.org>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] looking for a cryostat service tech. in the
Houston area.
Message-ID: <c4adea684afb4eca8ab82b00b078da57 at IDC-EX-11.shriners.cc>
Content-Type: text/plain; charset="us-ascii"
I am looking for a cryostat service tech in the Houston/Galveston area to do a PM.
Any recommendations would be appreciated.
Pam Hebert, HT(ASCP)
Shriners Hospitals for Children- Galveston, TX
------------------------------
Message: 15
Date: Wed, 28 Sep 2016 16:32:56 +0000
From: "Garcia, Lori, M.Sc." <lori.garcia at medtronic.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] EXAKT band saw blade
Message-ID:
<b7663f03f32a45d4808d0f44ea0e28f5 at mspm1bmsgm11a.ent.core.medtronic.com>
Content-Type: text/plain; charset="us-ascii"
Looking for an EXAKT band saw blade for a colleague due to a backorder. If anyone has one to loan out or sell, please contact me.
Thanks,
Lori
[CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com
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