[Histonet] Histonet Digest, Vol 154, Issue 22

Nasir Abbas shinwari_1 at yahoo.com
Wed Sep 28 03:39:40 CDT 2016


Dear Histoneters!Good DayMay I request you guys to share protocol of frozen tissues (mouse liver) for immunoflorescence assay. Lots of protocol are available online but i will highly appreciate the one that could lead me from tissue preparation towards staining procedure as I am really blank about frozen tissues and slides dealing. So I will be on standby for your response. Thanks in advance.
Sincerely Nasir Abbas
---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue Science Park Guangzhou, 510530, Guangdong P.R.ChinaCell: +86-1312 8296 614 

    On Wednesday, 28 September 2016, 1:12, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:
 

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Today's Topics:

  1. GMS (Martin, Gary)
  2. Re: Unprocessing tissue (Caroline Miller)
  3. Water for H&E Stainers? (P Sicurello)
  4. Re: Water for H&E Stainers? (Elizabeth Chlipala)


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Message: 1
Date: Mon, 26 Sep 2016 16:30:49 -0700
From: "Martin, Gary" <gmartin at marshallmedical.org>
To: <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] GMS
Message-ID:
    <6ED9D4252F278841A0593D3D788AF24C366E5E51 at mailsvr.MARSHMED.local>
Content-Type: text/plain;    charset="us-ascii"

I am having difficulties with a stain I've done many times ...GMS. The
light green is only staining the outer edges of the specimen. Any Ideas?


Thanks 

Gary



------------------------------

Message: 2
Date: Mon, 26 Sep 2016 16:49:14 -0700
From: Caroline Miller <mills at 3scan.com>
To: "Armstrong, Karoleigh T" <Karoleigh.Armstrong at armc.net>
Cc: "histonet at lists.utsouthwestern.edu"
    <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Unprocessing tissue
Message-ID:
    <CAFTHRQMn5=hqZoYF-FrJ-mF2agzEUP_gzwyjxNhGUx90hMkskA at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Not crazy at all, this is the kind of thing I was thinking someone would
have out there :)

I have used downey on hard tissue block faces before now too!

thanks so much for all your advice histonet :)

mills

On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Caroline,
>
> This may sound a little crazy,
>
> but I was working on some mummified tissue back in the 80's, (Mommy is
> old), and to deal with the dried out tissue we would give them,
>
> 1. A quick .5 percent Sodium Hydroxide soak, 5minutes
>
> 2. Wash is running water, 1 minute
>
> 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes
>
> 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes.
>
> 5. Wash with running water 2-3 minutes.
>
> Proscess your tissue as you usually would.
>
> They came out pretty darn good.
>
> KK Armstrong H.T. (ASCP)
>
>
> --------------------------------------------------------------------------
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297


------------------------------

Message: 3
Date: Tue, 27 Sep 2016 09:28:25 -0700
From: P Sicurello <patpxs at gmail.com>
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Water for H&E Stainers?
Message-ID:
    <CAPSjddYCETA1FLdSUFgwzCTaYSsQk6rXDrVTssqjmdwQAjUsPg at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Good Morning Everyone,



What type of water do you use with your automated H&E stainers?  House or
deionized/distilled?



We cannot buy one of the waterless, fancy schmancy H&E stainers at this
time.  L
Thank you in advance.

Sincerely,



Paula



Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.


------------------------------

Message: 4
Date: Tue, 27 Sep 2016 10:54:18 -0600
From: Elizabeth Chlipala <liz at premierlab.com>
To: P Sicurello <patpxs at gmail.com>,
    "'histonet at lists.utsouthwestern.edu'
    (histonet at lists.utsouthwestern.edu)"
    <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Water for H&E Stainers?
Message-ID:
    <14E2C6176416974295479C64A11CB9AE02BEEABA5B09 at SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

Paula

We have ours hooked up to tap water.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, September 27, 2016 10:28 AM
To: HistoNet
Subject: [Histonet] Water for H&E Stainers?

Good Morning Everyone,



What type of water do you use with your automated H&E stainers?  House or deionized/distilled?



We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.  L Thank you in advance.

Sincerely,



Paula



Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



*Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.  Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.  If you received this e-mail in error, please contact the sender and delete the material from any computer.
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------------------------------

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------------------------------

End of Histonet Digest, Vol 154, Issue 22
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