[Histonet] Unprocessing tissue

[Caroline Miller]

Not crazy at all, this is the kind of thing I was thinking someone would
have out there :)

I have used downey on hard tissue block faces before now too!

thanks so much for all your advice histonet :)

mills

On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Caroline,
>
> This may sound a little crazy,
>
> but I was working on some mummified tissue back in the 80's, (Mommy is
> old), and to deal with the dried out tissue we would give them,
>
> 1. A quick .5 percent Sodium Hydroxide soak, 5minutes
>
> 2. Wash is running water, 1 minute
>
> 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes
>
> 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes.
>
> 5. Wash with running water 2-3 minutes.
>
> Proscess your tissue as you usually would.
>
> They came out pretty darn good.
>
> KK Armstrong H.T. (ASCP)
>
>
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297