[Histonet] Unprocessing tissue from paraffin

Maria Mejia mbmphoto at gmail.com
Wed Sep 21 21:10:06 CDT 2016

Hi Millis,

Lets see if I’m understanding you correctly. 
1) You have unprocessed tissue. What kind of unprocessed tissue & how thick are the free-floating sections?
2) Are the tissue fixed? If so, with what? & For how long?
3) You want to stain the unknown whole mount using various histochemical stains or IHC stains?
4) Can you please explain why after staining you need to reprocess for paraffin? 
Cause once these mystery sections are processed you'll have to cut them using a rotary microtome.

I can maybe help you, but your going to have to provide more detail information. Also provide your
processing protocol.

Maria Mejia
Lead Histologist
Memory & Aging Center
> On Sep 21, 2016, at 12:38 PM, Caroline Miller via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> Hi there Histonetters!
> I am currently working through some issues of unprocessing tissue to then
> restain 'whole mount' with various stains, and then reprocess back to
> paraffin.
> I am running into issues of tissue being brittle and scratchy tissue after
> reprocessing and sectioning.
> Can anyone offer any advice on kinder dewaxing procedures and / or
> additives to the unprocessing / processing fluids that might help in the
> resulting piece of tissue?
> I have currently been putting it through the cleaning cycle on the
> processor, so standard xylene then alcohol treatment.
> thanks in advance for your advice!
> mills
> -- 
> Caroline Miller (mills)
> Director of Histology
> 3Scan.com
> 415 2187297
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