[Histonet] Unprocessing tissue from paraffin

[Caroline Miller]

Hi there Histonetters!

I am currently working through some issues of unprocessing tissue to then
restain 'whole mount' with various stains, and then reprocess back to
paraffin.

I am running into issues of tissue being brittle and scratchy tissue after
reprocessing and sectioning.

Can anyone offer any advice on kinder dewaxing procedures and / or
additives to the unprocessing / processing fluids that might help in the
resulting piece of tissue?

I have currently been putting it through the cleaning cycle on the
processor, so standard xylene then alcohol treatment.

thanks in advance for your advice!
mills




-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297