[Histonet] Histonet Digest, Vol 154, Issue 13 tissue loss

[Steve McClain]

Wendy
Develop your own data.
Check your early steps first. Tissue loss can be simple -a function of poor fixation, dirty processing or rush processing or slightly large tissue w slightly dirty reagents.

Airdry wet slides before annealing on hot plate. 
Check your baking/annealing. Some tissues require a bit more. 

We do not use that stainer.

Check your heat antigen retrieval against a manual retrieval.
Skin can handle 90 degrees for twice as long as 98. 98 degrees may cause excessive collagen loss. 

Steve A. McClain, MD

> On Sep 16, 2016, at 13:25, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:
> 
> Wendy