[Histonet] Movat Pentachrome

[Elizabeth Chlipala]

If you are not that concerned over the alcian blue portion of the movats you can perform an elastic trichrome - that's what we do here.  Mordant like you would in Bouins, and rather than stain with weigerts hemastoxylin, stain with the elastic portion of the VVG, differentiate like you would for elastic fibers and then continue on with the rest of the trichrome, it turns out great.  I have an SOP if anyone wants it.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

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Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Forest Blankenship via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 11:24 AM
To: 'histonet at lists.utsouthwestern.edu'
Subject: Re: [Histonet] Movat Pentachrome

Angela, the Movat is a wonderful thing for showing  loss of elastic fibers in emphysema and cardiovascular cases. We ran a ton of them in training because our lab/histo school was affiliated with the Texas Heart Institute.
Currently at the hospital I work at we use a Kit(Polyscientific)  for the VVG part of the stain and Saffron du Galantis for the counterstain. It smells great when gently heated.
As far as remarks about a trichrome being able to show the same thing; yeah if you also run a VVG on a serial section to show elastic fibers  and you lose the awesomeness that is the Movat.

Forest Blankenship, HTL(ASCP)
Histology Manager
Driscoll Children's Hospital
Corpus Christ, TX

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