[Histonet] Modified Movat's Pentachrome stain

[Rene J Buesa]

Do you have any contacts at any old histology lab, i.e., one that has been in operation for more than 50 years? You may find there iodine crystals and ask for a few grams. I used to have a 500 g bottle at my lab (which began in 1947).René 

    On Thursday, September 1, 2016 10:39 AM, Angela Lamberth <alamberth at lji.org> wrote:
 

 I would prefer to not substitute anything yet the procedure calls for a minimum of 2 grams of iodine which I simply cannot buy in the United States thanks to new DEA regulations.
On Thu, Sep 1, 2016 at 7:21 AM, Rene J Buesa <rjbuesa at yahoo.com> wrote:

Once you start substituting things in an original recipe, the outcome cannot be expected to be what the original recipe was supposed to deliver.Iodine crystals cannot be substituted by Lugol because, besides the iodine also contains its salts. and alcohol. They are two completely different things. This is the same as using Harris hematoxylin without mercury, you can keep calling it Harris, but it no longer will stain the same. Finally I do not see any advantages of Movat's pentachrome over a good Masson. Just my opinion but I think you are embarking in a complex process with little chance of finishing with the expected results.René 

    On Wednesday, August 31, 2016 9:53 PM, Angela Lamberth via Histonet <mailto:histonet at lists.utsouthwestern.edu> wrote:
 

 Hi Histonetters!

I’m gearing up to perform a pentachrome stain. I will be making this in
house and not using a kit. Through searching histonet, I’ve found a
protocol used by the Children’s Hospital of Philadelphia Pathology Core.
http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf


Two things:  Iodine crystals are out of the question. Can I replace that
with Lugol’s iodine solution or should I just substitute Weigert’s for the
hematoxylin in this protocol?


Also, saffron vs tartrazine vs Orange G stain.  There is a real difference
in price. Does anybody have a personal preference in terms of quality or
aesthetics? Is paying the extra money for saffron really worth it?


Thanks!

Angela



-- 
Angela Lamberth
Histology Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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-- 
Angela LamberthHistology Technician IIHistology Core LabLa Jolla Institute for Allergy & Immunology9420 Athena CircleLa Jolla, CA 92037