[Histonet] aniline oil/Holzer stain

Bryan Llewellyn llewllew at shaw.ca
Fri Oct 21 22:43:19 CDT 2016

Aniline oil is more commonly known just as aniline. Sigma list it for 
sale at: 

Bryan Llewellyn

Susan Bachus via Histonet wrote:
> I'm trying to locate aniline oil for the Holzer glial fiber stain.  I
> purchased what was listed as aniline oil from ENG, but the first hint
> that something was wrong was that it was incompatible with the
> chloroform also included in the differentiating solution.  Looking at
> the fine print on the bottle, I saw that it was actually "aniline oil,
> 2.5% aqueous", which I would have interpreted as only containing 2.5%
> water, except that it seemed to be completely aqueous (2.5% aniline?),
> inasmuch as it wouldn't mix with the chloroform.  I can't find any other
> source online.  I see that it is also called aminobenzine or
> phenylamine, but am also having trouble locating sources under those
> names.  I realize aniline oil is relatively toxic, but am using it under
> a fume hood.  I tried dipping slides separately in the (apparently
> aqueous) aniline and then chloroform, but still didn't get good
> differentiation.  Can anyone suggest a source or a substitute?  Many
> thanks for your help!   Susan
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