[Histonet] aniline oil/Holzer stain
susanbachus at verizon.net
susanbachus at verizon.net
Fri Oct 21 17:06:23 CDT 2016
I'm trying to locate aniline oil for the Holzer glial fiber stain. I
purchased what was listed as aniline oil from ENG, but the first hint that
something was wrong was that it was incompatible with the chloroform also
included in the differentiating solution. Looking at the fine print on the
bottle, I saw that it was actually "aniline oil, 2.5% aqueous", which I
would have interpreted as only containing 2.5% water, except that it seemed
to be completely aqueous (2.5% aniline?), inasmuch as it wouldn't mix with
the chloroform. I can't find any other source online. I see that it is
also called aminobenzine or phenylamine, but am also having trouble locating
sources under those names. I realize aniline oil is relatively toxic, but
am using it under a fume hood. I tried dipping slides separately in the
(apparently aqueous) aniline and then chloroform, but still didn't get good
differentiation. Can anyone suggest a source or a substitute? Many thanks
for your help! Susan
More information about the Histonet
mailing list