[Histonet] clip-on magnifiers

Wed Oct 19 08:32:36 CDT 2016

RE: clip-on magnifiers 
Try Ted Pella

Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  CDavis at che-east.org

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Subject: Histonet Digest, Vol 155, Issue 15

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Today's Topics:

   1. Clip-On Magnifiers for Embedding (Lisa Hamilton)
   2. Beta-Catenin (Algeo, Lacie A)
   3. Picrosirius Red (Bax, N.A.M.)

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Message: 1
Date: Mon, 17 Oct 2016 14:22:26 -0500
From: "Lisa Hamilton" <lhamilton at wcplaboratories.com>
To: <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Clip-On Magnifiers for Embedding
Message-ID: <000901d228ab$cdacac90$690605b0$@com>
Content-Type: text/plain;       charset="us-ascii"

In the past I have seen clip-on magnifiers that are used to embed tissue. Of
course now that I need  one I cannot remember what catalog I saw them in! I
was hoping someone might

know where I can find them.

Thank You

-Lisa

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Message: 2
Date: Mon, 17 Oct 2016 19:53:28 +0000
From: "Algeo, Lacie A" <Lacie.Algeo at providence.org>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Beta-Catenin
Message-ID:
        <24C4B3C167E5694887AB594C7602CE3A03CE1CCE at WN35104.or.providence.org>
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Hi All,
What are people using for Beta-catenin control tissue?  Is anyone using tissue that is known specifically to have a mutation for the beta-catenin gene?  If so, is this something that is purchased?
Thanks,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.algeo at providence.org<mailto:lacie.algeo at providence.org>

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Message: 3
Date: Tue, 18 Oct 2016 12:26:43 +0000
From: "Bax, N.A.M." <N.A.M.Bax at tue.nl>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Picrosirius Red
Message-ID:
        <67502D640FCB0641AACF2BE17717060C325A9D1D at xserver30b.campus.tue.nl>
Content-Type: text/plain; charset="us-ascii"

Dear Histosearch,

I just became a member and I hope you can provide me with advice. I'm really curious about the effect of not dissolving Direct Red 80 in 1.3% saturated Picric Acid but in 0.9-1.1% alkalimetric Picric acid solution. The reason why I'm curious is that I want to perform a staining on paraffin slides for Sirius Red and in my current lab they have Picrosirius red dissolved in 0.9-1.1% alkalimetric Picric acid, but the samples were I want to compare them to are stained in Picrosirius red made with the standard protocol with saturated Picric Acid.

I measured the pH of the Picrosirius Red made with 0.9-1.1% alkalimetric Picric acid and this is ~pH 2.0 and all the Direct Red is dissolved, so can I assume that the staining will be comparable to Picrosirius Red dissolved in saturated Picric acid?

Thank you very much in advance.

Best Noortje

Noortje AM Bax, PhD | Laboratory for Cell and Tissue Engineering  | Biomedical Engineering | Eindhoven University of Technology, P.O. Box 513, Groene Loper, Gebouw 15, Gemini-Zuid 4.108, 5600 MB Eindhoven,  The Netherlands | T: +31-40-2474830 | F: +31 40-2473744 |E-mail:  n.a.m.bax at tue.nl|<mailto:n.a.m.bax at tue.nl%7C>

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End of Histonet Digest, Vol 155, Issue 15
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