[Histonet] Vacuolated and torn?

Caroline Miller mills at 3scan.com
Thu Oct 6 18:39:07 CDT 2016

If it is a block issue you can also often see 'white' bits on the block
surface that correlate to the torn areas on the slide, try 'polishing' the
block at 1um until they go away, cool and then try sectioning again.

I am unsure how that relates to vacuolation - as I have usually seen that
referred to an intracellular event - and not to refer to a regular hole.

I also suggest having someone else cut a block and then compare.


On Thu, Oct 6, 2016 at 3:43 PM, Cartun, Richard via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Microtomy is so important, but often over-looked.  You might try giving
> the paraffin block(s) to different people and have them all cut H&Es, and
> then have your pathologist compare them.  If everyone's sections are
> suboptimal, then the problem is with fixation and/or processing.
> Richard
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology &
> Morphologic Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596
> (860) 545-2204 Fax
> -----Original Message-----
> From: Martin, Erin via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Thursday, October 06, 2016 9:31 AM
> To: histonet
> Subject: [Histonet] Vacuolated and torn?
> Good morning everyone!
> One of my pathologists says that we are having a problem with the tissue
> on the slides looking vacuolated and torn.  He is convinced it is from
> microtomy.  Anyone have any ideas?  I was thinking that it might be a
> processing issue.
> Thanks in advance!
> Erin Martin, Histology Supervisor
> UCSF  Dermatopathology and Oral Pathology Service
> 1701 Divisadero St, San Francisco, CA 94044
> 415-353-7248
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Caroline Miller (mills)
Director of Histology
415 2187297

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