[Histonet] Vacuolated and torn?
Richard.Cartun at hhchealth.org
Thu Oct 6 17:43:44 CDT 2016
Microtomy is so important, but often over-looked. You might try giving the paraffin block(s) to different people and have them all cut H&Es, and then have your pathologist compare them. If everyone's sections are suboptimal, then the problem is with fixation and/or processing.
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
80 Seymour Street
Hartford, CT 06102
(860) 545-2204 Fax
From: Martin, Erin via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, October 06, 2016 9:31 AM
Subject: [Histonet] Vacuolated and torn?
Good morning everyone!
One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn. He is convinced it is from microtomy. Anyone have any ideas? I was thinking that it might be a processing issue.
Thanks in advance!
Erin Martin, Histology Supervisor
UCSF Dermatopathology and Oral Pathology Service
1701 Divisadero St, San Francisco, CA 94044
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