[Histonet] Vacuolated and torn?

Cartun, Richard Richard.Cartun at hhchealth.org
Thu Oct 6 17:43:44 CDT 2016

Microtomy is so important, but often over-looked.  You might try giving the paraffin block(s) to different people and have them all cut H&Es, and then have your pathologist compare them.  If everyone's sections are suboptimal, then the problem is with fixation and/or processing.


Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Martin, Erin via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, October 06, 2016 9:31 AM
To: histonet
Subject: [Histonet] Vacuolated and torn?

Good morning everyone!

One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn.  He is convinced it is from microtomy.  Anyone have any ideas?  I was thinking that it might be a processing issue.

Thanks in advance!

Erin Martin, Histology Supervisor
UCSF  Dermatopathology and Oral Pathology Service
1701 Divisadero St, San Francisco, CA 94044

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