[Histonet] Cytology/Histology Staining Question (Mullen, Mary)

[O'Donnell, Lynn M.]

This is the CAP regulation that talks about cross contamination.


CYP.04150 Cross-Contamination Phase I
There is a written procedure to prevent cross-contamination of specimens during
processing and staining.
NOTE: Procedures must prevent cross-contamination between gynecologic and non-gynecologic
specimens.
Also, procedures must prevent contamination among non-gynecologic cases when highly
cellular specimens are processed. Methods to minimize this potential problem may include
cytocentrifuge, filter, and monolayer preparations. Direct smears made from the sediment of
highly cellular cases should be stained after the other cases, and the staining fluids must be
changed or filtered between each of the highly cellular cases. One procedure to detect highly
cellular specimens is to use a toluidine blue, or other rapid stain, on a wet preparation. One
procedure to detect possible contamination is to insert a clean blank slide in each staining run
and examine it for contamination.
REFERENCES
1) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement
amendments of 1988; final rule. Fed Register. 2003(Jan 24):7169 [42CFR493.1274(b)(2-3)]




Lynn M. O'Donnell, CT (ASCP), MHA l Technical Specialist, Cytology
Danbury Hospital l lynn.o'donnell at wchn.org
tel: 203-739-6704  Fax: 203-739-6034




-----Original Message-----
From: T H via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, May 10, 2016 13:46
To: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary)

Hey Mary,

The problem is not the machine, it is the reagents sharing that is the issue.  You can use different reagents and protocols for the Histo and Cyto slides and on the same instrument.  You might even get away with changing your Alcohols and filtering everything else and see how that works.  Try it and run some blank slides through the stainer and see if anything is there from the cytology specimens.

I would personally have two separate sets of staining reagents to be on the safe side.

Good luck!!

Tim


Message: 7
Date: Tue, 10 May 2016 14:54:55 +0000
From: "Mullen, Mary" <mullenmk at mail.magee.edu>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Cytology/Histology Staining Question
Message-ID:
        <374DC72E6B29D44086F8FF3289351B2508823897 at MSXMBXNSPRD39.acct.upmchs.net>

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Hello all,



I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA



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