[Histonet] Cytology/Histology Staining Question (Mullen, Mary)

[T H]

Hey Mary,

The problem is not the machine, it is the reagents sharing that is the issue.  You can use different reagents and protocols for the Histo and Cyto slides and on the same instrument.  You might even get away with changing your Alcohols and filtering everything else and see how that works.  Try it and run some blank slides through the stainer and see if anything is there from the cytology specimens.

I would personally have two separate sets of staining reagents to be on the safe side.

Good luck!!

Tim


Message: 7
Date: Tue, 10 May 2016 14:54:55 +0000
From: "Mullen, Mary" <mullenmk at mail.magee.edu>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Cytology/Histology Staining Question
Message-ID:
        <374DC72E6B29D44086F8FF3289351B2508823897 at MSXMBXNSPRD39.acct.upmchs.net>

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Hello all,



I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA