[Histonet] Cytology/Histology Staining Question

[Hannen, Valerie]

Mary,

We run both protocols on the same stainer, however, each protocol has it own set of reagents except for the common water wells and the two xylenes at the end before the final xylene.  We have the Leica ST 5020, which has 36 wells not including the 2 oven wells.

Hope this helps.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com
www.parrishmed.com


-----Original Message-----
From: Mullen, Mary via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, May 10, 2016 10:55 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cytology/Histology Staining Question

Hello all,



I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols).



What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues?



We only run non-gyn cytology, all gyn cytology is sent out.







Thanks,



Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA

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