[Histonet] PAS Stain

[Seeley, Heather]

We have one girl that always spits on our slide! :) works great!

HEATHER SEELEY, HT(ASCP)
Histotech
803-985-4676 OFFICE
803-327-7598 FAX


________________________________________
From: Bob Richmond [rsrichmond at gmail.com]
Sent: Thursday, May 05, 2016 2:10 PM
To: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

Amylase (diastase) for the PAS stain queries:

Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a few seconds.

He notes that alpha amylase is preferred. I'd go with the cheapest one in
the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
offers a heat-stable alpha amylase.

Bob Richmond
Samurai Pathologist
Maryville TN