[Histonet] PAS Stain

[Rene J Buesa]

As I see it, there are 3 main objections about using human saliva as an amylase source.In order of importance they are:1- you will never know the actual concentration of the amylase and this will produce reproducibility problems.2- along with the saliva you will introduce bacteria that may end being stained and can cause misdiagnoses.3- it is absolutely disgusting.René 

    On Thursday, May 5, 2016 2:26 PM, Bob Richmond via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 Amylase (diastase) for the PAS stain queries:

Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a few seconds.

He notes that alpha amylase is preferred. I'd go with the cheapest one in
the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
offers a heat-stable alpha amylase.

Bob Richmond
Samurai Pathologist
Maryville TN
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