[Histonet] IHC positive controls

[Morken, Timothy]

Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they  are fully isolated from air, which paraffin does, but once cut, they are exposed. That happens in the fridge as well and I'm not sure refrigeration helps much. I'm guessing that you don't do a lot of these, so you don't want to cut a lot of slide and have them sitting around for months waiting for use. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-----Original Message-----
From: Cindy Bulmer via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Monday, May 02, 2016 10:43 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls


Hello Histoland,
 
I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?
 
1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. 
     then put slides in refrigerator for future use.
2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and put slides
     directly in refrigerator for future use.
3)  Cut "fresh" every time they order the Ab.
 
Thank you,
Cindy


Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX




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