[Histonet] Processing FFPE tissue without alcohol

[Dessasau III, Evan]

Will they be able to do IHC stains?

-----Original Message-----
From: Wanda Shotsberger Gray via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Saturday, March 26, 2016 12:56 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Processing FFPE tissue without alcohol


While the tissue will still go through alcohol, have you considered preserving the fat with osmium tetroxide prior to routine processing? This turns the fat black, but it is retained in the tissue.


Hi Histonet, is there a way to process tissue for paraffin embedding without using alcohol? One of the labs that send their processing to us will be doing a study examining fat. I told them if they want to look at the fat they will have to cut frozen sections but I'm being ask again about processing without the alcohol. So I said I would ask around.

Please let me know what you think.

Thank you for your help!

E-van



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