[Histonet] Processing FFPE tissue without alcohol

[Wanda Shotsberger Gray]

While the tissue will still go through alcohol, have you considered 
preserving the fat with osmium tetroxide prior to routine processing? This 
turns the fat black, but it is retained in the tissue.


Hi Histonet, is there a way to process tissue for paraffin embedding 
without using alcohol? One of the labs that send their processing to us 
will be doing a study examining fat. I told them if they want to look at 
the fat they will have to cut frozen sections but I'm being ask again about 
processing without the alcohol. So I said I would ask around.

Please let me know what you think.

Thank you for your help!

E-van



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