[Histonet] Porcessing FFPE tissue without alcohol??

[Hobbs, Carl]

Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation)
Processing to Pwax as usual.
Basically, you will see lipids as black ( oxidised osmium)
That's the only way to demonstrate solvent- soluble lipids, using Pwax processing.
Sure, there are caveats but, in the main...it will be Ok, imho.
I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve.



  
 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813