[Histonet] Porcessing FFPE tissue without alcohol??

[Rene J Buesa]

To embed the tissues with paraffin you HAVE TO dehydrate the tissue. This is usually done with either ethanol of 2-propanol but essentially all dehydrants will remove fat so you are right, the way to go is going frozen sections.René 

    On Thursday, March 24, 2016 2:24 PM, "Dessasau III, Evan via Histonet" <histonet at lists.utsouthwestern.edu> wrote:
 

 Hi Histonet,  is there a way to process tissue for paraffin embedding without using alcohol?                        One of the labs that send their processing to us will be doing a study examining  fat.  I told them if they want to look at the fat they will have to cut frozen sections but I'm being ask again about processing without the alcohol.  So I said I would ask around.
Please let me know what you think.
Thank you for your help!
E-van

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