[Histonet] Processing issues

Tue Mar 22 11:22:47 CDT 2016

Not knowing what issues you have, I suggest you look to your samples for processing. Reduce thickness and the shorter times will work. 2-2.5 mm thick. Standardize fixation before placing in tissue processor. What time is the processor started and what time does the tech remove? You may be able to reduce delay and increase alcohols. Specimen thickness will reduce the the time for solution to move through the tissue sample and allow exchange of solution. There are many options, but be more specific about the problem and you can narrow the changes

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________________________________
From: Charles Riley via Histonet<mailto:histonet at lists.utsouthwestern.edu>
Sent: ‎3/‎22/‎2016 8:57 AM
To: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Processing issues

We are having issues with our tissue processing. We use the thermo shandon
excelsior processor. All steps have agitation and under vacuum. Our routine
process is  as follows

1. Formalin   1hr
2. Formalin   1hr
3. 75% alcohol 30 mins
4.85% alcohol 30 mins
5. 95% alcohol 30 mins
6.95% alcohol 30 mins
7. 100% alcohol 30 mins
8. 100% alcohol 30 mins
9 xylene 30 mins
10. xylene 30 mins
11 xylene 30 mins
12. Wax for 30 mins
13. Wax 30 mins
14. Wax 30 minutes.

Can anyone give any suggestions for altering this process to work better? I
know the process should probably be longer however medical director does
not want to delay turn around time. If possible keep the process to under 9
hours. If this isn't conducive to consistent processing please explain why
so  I can show my superiors to give myself some leverage to change things
--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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