[Histonet] Processing issues

[Mca Werdler]

Hey Charles,

Why are the first 2 steps of formalin that long? If you make sure your
tissue is well fixated, then there is no need for such long fixation in the
machine. You could use step 2 for just washing the formalin out, so for
only like 5 minutes. You could use that time, to add to other chemicals.

Hope it works for you.

Maarten Werdler

2016-03-22 9:26 GMT-06:00 Charles Riley via Histonet <
histonet at lists.utsouthwestern.edu>:

> We are having issues with our tissue processing. We use the thermo shandon
> excelsior processor. All steps have agitation and under vacuum. Our routine
> process is  as follows
>
> 1. Formalin   1hr
> 2. Formalin   1hr
> 3. 75% alcohol 30 mins
> 4.85% alcohol 30 mins
> 5. 95% alcohol 30 mins
> 6.95% alcohol 30 mins
> 7. 100% alcohol 30 mins
> 8. 100% alcohol 30 mins
> 9 xylene 30 mins
> 10. xylene 30 mins
> 11 xylene 30 mins
> 12. Wax for 30 mins
> 13. Wax 30 mins
> 14. Wax 30 minutes.
>
> Can anyone give any suggestions for altering this process to work better? I
> know the process should probably be longer however medical director does
> not want to delay turn around time. If possible keep the process to under 9
> hours. If this isn't conducive to consistent processing please explain why
> so  I can show my superiors to give myself some leverage to change things
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
> Doctors Pathology Services, Dover DE
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