[Histonet] Plastic coverslip Loosening

[Helen Fedor]

Thank you for your help. This is encouraging. 

Helen

-----Original Message-----
From: Glover, Kimberly [mailto:kimberly.glover at polysciences.com] 
Sent: Tuesday, March 22, 2016 11:38 AM
To: Helen Fedor <hfedor at jhmi.edu>
Cc: histonet <histonet at lists.utsouthwestern.edu>
Subject: RE: Plastic coverslip Loosening

Helen,

Submerge the slides with coverslip loosely attached in fresh xylene to reactivate the film resin. Allow to sit in xylene for approximately 1 minute. Afterwards remove slide and press down on a paper towel. This action will allow the film to reattach to the slide while pressing out any excess xylene from the slide. 

As for the film (containing tissue) that has completed detached from the slide, place the film against a clean slide and place in a slide basket. Submerge slide in fresh xylene and proceed with same procedure above. 

Sometimes the issue of loosening or lifting of film is seen over time when the coverslipped slides are placed in temperature/humidity conditions that are contrary to the manufacturer's recommendations. Check packaging for storage conditions recommended.

I hope this helps.

Kimberly A. Glover 
Life Sciences Product Manager
Polysciences, Inc.
400 Valley Road
Warrington, PA 18976
www.polysciences.com




-----Original Message-----
From: Helen Fedor [mailto:hfedor at jhmi.edu] 
Sent: Tuesday, March 22, 2016 11:06 AM
To: Glover, Kimberly
Subject: RE: Plastic coverslip Loosening

The film.

-----Original Message-----
From: Glover, Kimberly [mailto:kimberly.glover at polysciences.com] 
Sent: Tuesday, March 22, 2016 10:25 AM
To: Helen Fedor <hfedor at jhmi.edu>
Subject: RE: Plastic coverslip Loosening

Are you using plastic coverslipping film or the plastic coverslips?

Kimberly

-----Original Message-----
From: Helen Fedor via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, March 22, 2016 9:45 AM
To: HISTONET (histonet at lists.utsouthwestern.edu)
Subject: [Histonet] Plastic coverslip Loosening

Hello, I did try and search the archives to see if there was any advice on this but didn't find an answer.

Do you know of a good way to fix H&E slides that had plastic coverslips where the coverslip is lifting from the slide and in some cases coming completely off? In all cases the tissue is on the coverslip.

Thanks in advance.



Helen L. Fedor


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