[Histonet] PAS Question

[Marcum, Pamela A]

We have a problem with the cell membranes separating on our bone marrow slides on our PAS slides done daily.  We have tried everything to fix this and stop the problem.  We have tried manual staining, bulk staining on the Leica Stainer and the Dako Artisan and all have the same problem.  I am at a loss and the only thing left is the temperature of oven we dry slides in set at 65C.  We do up 38 bone marrows every night so this is critical for us.  If anyone can help please give us advise.

Pam

----------------------------------------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.