[Histonet] using FFPE positive control for frozen tissue IHC?

[Elizabeth Chlipala]

There are many companies that sell frozen tissue both normal and diseased.  Frozen IHC does not normally require retrieval most FFPE IHC does.  I don't think it would be good practice to do what you are suggesting.

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Preiszner, Johanna via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Monday, June 27, 2016 8:48 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] using FFPE positive control for frozen tissue IHC?

Hi,

We have difficulty obtaining frozen tissue to use as positive control when we do IHC on frozen sections...

Is it allowed to use FFPE sections for this purpose? If not, can someone explain to me why not? Is it wrong by principle or prohibited by regulations? If it's the regulations, can someone give me a reference, please?

I have not seen an antibody that works on FFPE tissue and refuses to work on frozen tissue...And the manufacturers always provide info about the type of tissue the antibody would work with.

Thank you,
Hanna Preiszner
ETSU/QCOM
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