[Histonet] using FFPE positive control for frozen tissue IHC?

[Preiszner, Johanna]

Hi,

We have difficulty obtaining frozen tissue to use as positive control when we do IHC on frozen sections...

Is it allowed to use FFPE sections for this purpose? If not, can someone explain to me why not? Is it wrong by principle or prohibited by regulations? If it's the regulations, can someone give me a reference, please?

I have not seen an antibody that works on FFPE tissue and refuses to work on frozen tissue...And the manufacturers always provide info about the type of tissue the antibody would work with.

Thank you,
Hanna Preiszner
ETSU/QCOM