[Histonet] Marking Tissues with Eosin

[Cynthia Robinson]

We use safranin at the grossing station and it is a dark pink at embedding. Works really well in our hands. Added plus is no fluorescent issues that you can have with eosin.

Cindi

________________________________________
From: Cindy Bird via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Thursday, June 23, 2016 4:20 PM
To: Mca Werdler
Cc: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Marking Tissues with Eosin

We place a small drop of concentrate straight on tissue.

Sent from my iPhone

> On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> Dear Rebecca,
>
> Yes this is possible. just don't use a too strong concentration. The eosin
> should give a slight pink color on the tissue after processing and after
> embedding.
>
> Good luck,
>
> Maarten
>
> 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
> histonet at lists.utsouthwestern.edu>:
>
>> I had a biopsy today that was nearly impossible to see on the sponges
>> during embedding or in the block.  I've heard mention of marking these with
>> eosin to make them easier to see.  Has anyone done this?  Or do you use
>> some other type of marking dye for this purpose?
>> Thanks for your input!
>> Rebecca
>>
>> Rebecca Ashley
>> Histotechnologist
>> Wyoming State Vet Lab
>> 1174 Snowy Range Rd.
>> Laramie, WY 82070
>> Phone: 307-766-9946
>> Fax: 307-721-2051
>>
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