[Histonet] Air Bubbles

[Barry Rittman]

There might be air in the mounting media that gives rise to bubbles when
drying starts to occur. One method to try is to use an ultrasonic bath  to
remove the air from the bottle containing the mounting media  before using.
We used to do this with aqueous mounting media.
Barry

On Fri, Jun 10, 2016 at 9:15 AM, Karen Pfaff via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

>
>
>
> There might not be enough mounting media placed on slide. So when slide is
> wet, there are no air bubble but after a couple of days the xylene or
> xylene substitute will evaporate and leave you with patchy areas. Has there
> been someone new coverslipping? I have been one of those people that put
> less mounting media on because the stage of the microscope stays cleaner,
> however, I discovered that I get air bubbles after it dries. Try putting a
> line of mounting media down the entire slide not just 2 or 3 drops. Might
> help.Karen PfaffLead HT Skin Cancer CenterMohs lab
>
> Sent from my U.S. Cellular® Smartphone
>
> -------- Original message --------
> From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
> Date: 06/10/2016  9:00 AM  (GMT-06:00)
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Air Bubbles
>
> Hello everyone,
>
> Recently we have been having an issue with a majority of our slides having
> air bubbles under the coverslips. When they are originally coverslipped
> there are no bubbles and any present are pushed out and the sides are all
> wiped dry. We have not changed any reagent or processes in over two years.
> Can anyone offer suggestions as to why this might be occurring or tips to
> fix the issue?
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
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