[Histonet] Air Bubbles

[Karen Pfaff]

There might not be enough mounting media placed on slide. So when slide is wet, there are no air bubble but after a couple of days the xylene or xylene substitute will evaporate and leave you with patchy areas. Has there been someone new coverslipping? I have been one of those people that put less mounting media on because the stage of the microscope stays cleaner, however, I discovered that I get air bubbles after it dries. Try putting a line of mounting media down the entire slide not just 2 or 3 drops. Might help.Karen PfaffLead HT Skin Cancer CenterMohs lab

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-------- Original message --------
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
Date: 06/10/2016  9:00 AM  (GMT-06:00)
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Air Bubbles

Hello everyone,

Recently we have been having an issue with a majority of our slides having
air bubbles under the coverslips. When they are originally coverslipped
there are no bubbles and any present are pushed out and the sides are all
wiped dry. We have not changed any reagent or processes in over two years.
Can anyone offer suggestions as to why this might be occurring or tips to
fix the issue?

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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