[Histonet] Distilled Water vs DI water for IHC and H&E staining

Wheelock, Timothy R. twheelock at mclean.harvard.edu
Thu Jul 28 08:25:43 CDT 2016

Hi Charles:

Many years ago I was using deionized water to do the Bielschowsky silver stain.
The stains were coming out way too dark, with black-brown precipitate on the sections.
When I inherited a double-distilled water filtration system, that problem disappeared.
However, silver stains tend to be sensitive to all sorts of things.
I don't know if the same holds true for immunostains, since I don't do them myself (another lab does them for us).
When I do my Luxol Fast Blue (myelin)-Hematoxylin-Eosin stain, I just use tap water or all my washes.
I make my solutions for the stain with double-distilled water, but I am not sure if that is necessary.
I hope that this helps.


Tim Wheelock
Harvard Brain Tissue Resource Center
McLean Hospital
Belmont, MA

-----Original Message-----
From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, July 28, 2016 7:54 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining

Does anyone know if there is a difference in staining results when using DI water versus Distilled water? Our lab is trying to get away from the DI system we have as it is becoming expensive to maintain and we were looking to see if we could just use a home drinking filter with UV lighting for our water supply. Please let me know any thoughts or concerns you might have with this idea


Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Histonet at lists.utsouthwestern.edu

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