[Histonet] Histonet Digest, Vol 152, Issue 5
Martin, Gary
gmartin at marshallmedical.org
Thu Jul 7 12:22:23 CDT 2016
We are also interested in a reasonablly price and simple document control system.
Thanks
Gary
-----Original Message-----
From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu]
Sent: Thursday, July 07, 2016 10:00 AM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 152, Issue 5
Send Histonet mailing list submissions to
histonet at lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request at lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner at lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Document control system: (J B)
2. Re: Samples not staining with Hematoxylin or primary
(Teri Johnson)
3. Re: Media Lab (Lester Raff MD)
4. Re: Media Lab (J B)
5. MITF (Baldwin, Kathy)
6. Samples not staining with Hematoxylin or primary
(Anna Huntley Coffey)
7. CWD testing (Johnson, Carole)
8. Why is Auromine heated in Histology, but not in Microbiology
(Eddie Martin)
9. Re: MITF (Eddie Martin)
10. Special Stain (Diana Martinez-Longoria)
----------------------------------------------------------------------
Message: 1
Date: Wed, 06 Jul 2016 17:23:18 +0000
From: J B <craigak12 at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Document control system:
Message-ID:
<CANyXu7DTTkWNYNF8ABaAQG9q=3YBxgboPOBEP7zrus8UMMQ7TQ at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Does anyone have experience with Media Labs document control system? I am
looking for good software for documentation control. Any feedback is
appreciated.
Sincerely,
JB
------------------------------
Message: 2
Date: Wed, 6 Jul 2016 17:55:30 +0000
From: Teri Johnson <tejohnson at genoptix.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Samples not staining with Hematoxylin or
primary
Message-ID:
<6e205ab6e3544c63abb55c8fec2da201 at PHUSCB-SP37MB01.genoptix.org>
Content-Type: text/plain; charset=WINDOWS-1252
Anna wrote:
I'm curious as to why we heat the Auramine Rhodamine when we are staining tissue sections but our Micro lab stains at room temp. It is because "it's always been done that way"?
Thanks group,
Angie
Hi Anna,
If your dispensers are working properly, I would bet it's your slides. There are known issues with overly hydrophobic slides causing issues on the Ventana platform. They recommend you use Plus (+) slides manufactured from Erie Scientific. There may be others I am not aware of, but we use these slides for all IHC sectioned here and have had no issues on our Ventanas.
Good luck!
Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954 (office)
760.516.6201 (fax)
________________________________
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message.
------------------------------
Message: 3
Date: Wed, 6 Jul 2016 18:12:32 +0000
From: Lester Raff MD <LRaff at uropartners.com>
To: "Histonet at lists.utsouthwestern.edu"
<Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Media Lab
Message-ID:
<6347C6D2B080534F9B5C2B08436DCFAF10E78B6A at COLOEXCH01.uropartners.local>
Content-Type: text/plain; charset="us-ascii"
We have been using MediaLab for several years, beginning with their online courses and progressing to Document Control and InspectionProof. We are awaiting our first CAP inspection in which I expect the inspectors to be able to get virtually all the information they need from our MediaLab site. Also, customer support is great!
It took a bit of time for the lab staff to accept the concept of online manuals and SOP's but in the long run it is a big win.
--------------------------
Latest blog post: http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/
--------------------------
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
-----Original Message-----
From: J B via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, July 06, 2016 12:23 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Document control system:
Does anyone have experience with Media Labs document control system? I am looking for good software for documentation control. Any feedback is appreciated.
Sincerely,
JB
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
------------------------------
Message: 4
Date: Wed, 06 Jul 2016 18:47:56 +0000
From: J B <craigak12 at gmail.com>
To: Lester Raff MD <LRaff at uropartners.com>,
"Histonet at lists.utsouthwestern.edu"
<Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Media Lab
Message-ID:
<CANyXu7C6Fn0SpwDoy1sLXnE9gAAjajK12UU24BVak1RcBwuvoA at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Thank you for the feedback. Was it hard or time consuming to upload all
procedures? I will only have a few weeks before my CAP window starts.
Thank you,
JB
On Wed, Jul 6, 2016, 11:26 AM Lester Raff MD via Histonet <
histonet at lists.utsouthwestern.edu> wrote:
>
>
> We have been using MediaLab for several years, beginning with their online
> courses and progressing to Document Control and InspectionProof. We are
> awaiting our first CAP inspection in which I expect the inspectors to be
> able to get virtually all the information they need from our MediaLab site.
> Also, customer support is great!
>
>
>
> It took a bit of time for the lab staff to accept the concept of online
> manuals and SOP's but in the long run it is a big win.
>
>
>
> --------------------------
>
>
>
> Latest blog post:
> http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/
>
>
>
> --------------------------
>
> Lester J. Raff, MD MBA
>
> UroPartners
>
> Medical Director Of Laboratory
>
> 2225 Enterprise Dr. Suite 2511
>
> Westchester, Il 60154
>
> Tel: 708-486-0076
>
> Fax: 708-492-0203
>
>
>
> -----Original Message-----
> From: J B via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Wednesday, July 06, 2016 12:23 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Document control system:
>
>
>
> Does anyone have experience with Media Labs document control system? I am
> looking for good software for documentation control. Any feedback is
> appreciated.
>
>
>
> Sincerely,
>
>
>
> JB
>
> _______________________________________________
>
> Histonet mailing list
>
> Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu
> >
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 5
Date: Wed, 6 Jul 2016 20:12:30 +0000
From: "Baldwin, Kathy" <sbaldwin at mhhcc.org>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] MITF
Message-ID: <1163fb0a386e4a22afebbd3d8d841c67 at exch02.mhhcc.org>
Content-Type: text/plain; charset="us-ascii"
Hi Histonetters
Was wondering if anyone has had any experience with MITF what controls would you use I have a fairly new pathologist that wants us to use Nevus' instead on melanoma and half the cases are staining and the other half are not what is your experience? Any help would be greatly appreciated!
Thanks
S. Kathy Baldwin
Histology/Cytology Supervisor
Memorial Hospital and Health Care Center
800 West 9th St.
Jasper, Indiana 47546
Office 812-996-0210
Fax 812-996-0232
Cell 812-887-3357
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
------------------------------
Message: 6
Date: Wed, 6 Jul 2016 16:14:26 -0400
From: Anna Huntley Coffey <annamhuntley at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Samples not staining with Hematoxylin or primary
Message-ID:
<CAHnCdCfehTkEyMPEVg7yV8VvU7L8tM9GWsmPVd7ESgntHzDwpw at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Thanks so much to everyone who responded! A couple people have suggested
that this issue may be due to a disruption to the charge on the slide (due
to high temp baking, water bath additives, etc) which causes a hydrophobic
barrier to form and prevents reagent from reaching the tissue. This sounds
very convincing to me, but we have found that repeated antigen retrieval
using a pressure cooker (our standard retrieval for this particular
primary) has allowed 2 of the 3 slides to stain with both DAB and
Hematoxylin. Would repeated retrieval be able to reverse the slide charge
back to normal?
I'm having an issue with some recent samples and was wondering if anyone
else has experienced the same thing...I stained some samples with a
previously optimized protocol for anti-human Granzyme B on the Ventana
Benchmark and about 1/3 of my samples did not stain at all with either
GranzB or Hematoxylin. My controls and samples that did stain positive for
GranzB look fine and the machine has been recently serviced and working
well with other runs. I'd really appreciate your input if you have any
advice or suggestions on what could have gone wrong.
Thanks,
Anna
------------------------------
Message: 7
Date: Wed, 6 Jul 2016 21:25:16 +0000
From: "Johnson, Carole" <cjohnson at nmda.nmsu.edu>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] CWD testing
Message-ID:
<F00D04066B62F04480A441B7460C17B5EC113EC4 at EX-MBX-P1.ACN.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
I would like to hear from people who are doing CWD testing on biopsies (tonsil or rectal). Are you placing a disclaimer on results, and if so do you include the number of follicles present in the specimen? You can answer privately at: cjohnson at nmda.nmsu.edu<mailto:cjohnson at nmda.nmsu.edu> Thanks for your input
Carole L. Johnson, HT(ASCP)cm, QIHC
New Mexico Department of Agriculture
Veterinary Diagnositc Services
1101 Camino de Salud, NE
Albuquerque, NM 87101
505.383.9299
Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act.
------------------------------
Message: 8
Date: Wed, 6 Jul 2016 23:45:01 -0500
From: Eddie Martin <edmartin26 at gmail.com>
To: "histonet (histonet at lists.utsouthwestern.edu)"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Why is Auromine heated in Histology, but not in
Microbiology
Message-ID: <077D5DFB-890A-4ACD-89F9-8EC66EB3B8B1 at gmail.com>
Content-Type: text/plain; charset=us-ascii
Hi Angie,
The simplest answer, would be to save time. Whether heated, or microwaved at 80 power for 45 seconds, would be quicker than flooding the slide and letting stand for extended periods of time.
------------------------------
Message: 9
Date: Thu, 7 Jul 2016 00:46:25 -0500
From: Eddie Martin <edmartin26 at gmail.com>
To: "histonet (histonet at lists.utsouthwestern.edu)"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] MITF
Message-ID: <24F35FB0-A7E9-4261-BDE0-27B4F1AD85B1 at gmail.com>
Content-Type: text/plain; charset=us-ascii
Hi Kathy,
The Journal of Investigative Dermatology had an article in their December 2011 Volume 131 issue 12 on Human Cutaneous Melanomas lacking MITF and melanocyte differentiation. According to the article, Melanomas could be MITF and Melan-A negative, if the Melanomas were TAM tyrosine kinase receptor positive. In this particular study, 38% of the total Cutaneous Melanomas were Axl+ (member of TAM), and none of the Axl+ were MITF and Melan-A positive.
That being said, it may explain why some of your cutaneous melanomas are not MITF positive. Known internal control tissue for MITF includes epithelioid tissue, mast cells, reactive histiocytes and osteoclasts stain intensely for MITF. A nevus would also be an ideal control tissue, as would any other perivascular epithelioid tumor.
Best,
Eddie Martin, HTL, HT(ASCP), QIHC
------------------------------
Message: 10
Date: Thu, 7 Jul 2016 12:49:29 +0000
From: Diana Martinez-Longoria <Diana.Martinez-Longoria at ecrmc.org>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Special Stain
Message-ID:
<12B71261212BE94BB9FB7735484B9FA178C8B208 at EXMBX01.ecrmc.ci.el-centro.ca.us>
Content-Type: text/plain; charset="us-ascii"
Good morning everyone,
I was wondering if anyone can help us in regards to special stain contamination. We will like to know if contamination can happen as a result of reagents touching the writing of a marking pen on a slide? For example we are manually running a slide that has the typical marking pen writing on the slide for identification and we are doing a silver stain, will it contaminate and not give an accurate result? I know this might be a weird question, but any information, resources, documentation you can direct me to would be very much appreciated. Please email me privately at dmlongoria at ecrmc.org<mailto:dmlongoria at ecrmc.org>. Thank you!
ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.
Â
------------------------------
Subject: Digest Footer
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
End of Histonet Digest, Vol 152, Issue 5
****************************************
More information about the Histonet
mailing list