[Histonet] Samples not staining with Hematoxylin or primary
tejohnson at genoptix.com
Wed Jul 6 12:55:30 CDT 2016
I'm curious as to why we heat the Auramine Rhodamine when we are staining tissue sections but our Micro lab stains at room temp. It is because "it's always been done that way"?
If your dispensers are working properly, I would bet it's your slides. There are known issues with overly hydrophobic slides causing issues on the Ventana platform. They recommend you use Plus (+) slides manufactured from Erie Scientific. There may be others I am not aware of, but we use these slides for all IHC sectioned here and have had no issues on our Ventanas.
Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
SAN5, Rm. 2005
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