[Histonet] Nuclear bubbling artifact

[Gudrun Lang]

I've read about a group, that observed living cells during the
fixation-process. They saw bubbling in the first period of contact and
penetration of formaldehyde. After a certain time the bubbles disappeared
again. 
Along this observation for me bubbles are a sign of too short fixation.

Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: Teri Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Freitag, 29. Jänner 2016 19:49
An: 'histonet at lists.utsouthwestern.edu'
Betreff: Re: [Histonet] Nuclear bubbling artifact

Hi James,

Nuclear bubbling artifact is most commonly seen in formalin fixed epithelial
cells, and GI biopsies are among those samples that are particularly
susceptible to it. It has been linked to inadequate fixation and also to
heating of slides prior to staining without complete air-drying of the
tissues. I would recommend cutting the block again, air drying the slides
for a time before using heat to melt the wax prior to H&E stain and see if
the artifact persists.

http://www.cap.org/apps/docs/proficiency_testing/nuclear_bubbling.pdf

Best wishes,
Teri Johnson


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