[Histonet] Nuclear bubbling artifact

[Teri Johnson]

Hi James,

Nuclear bubbling artifact is most commonly seen in formalin fixed epithelial cells, and GI biopsies are among those samples that are particularly susceptible to it. It has been linked to inadequate fixation and also to heating of slides prior to staining without complete air-drying of the tissues. I would recommend cutting the block again, air drying the slides for a time before using heat to melt the wax prior to H&E stain and see if the artifact persists.

http://www.cap.org/apps/docs/proficiency_testing/nuclear_bubbling.pdf

Best wishes,
Teri Johnson


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