[Histonet] Tissue Arrays

[Amos Brooks]

Hi,
     My experience with microarrays is that they are sometimes a bit
complicated to work with depending on the way they are constructed and the
platform they are used on. Often these slides are dipped in paraffin to
help preserve them. When this happens they need considerably longer
deparaffinization. They also sometimes are not floated on a waterbath to
transfer them to the slide but by a tape transfer process. The tape often
interferes in IHC staining and especially on Ventana platforms that make
use of the liquid coverslips. If you can try to get microarrays that are
floated like regular sections, make sure you give them a good long soak in
xylene, and perhaps increase the volume per slide of the antibodies.

Best of luck,
Amos


On Wed, Jan 27, 2016 at 1:00 PM, <histonet-request at lists.utsouthwestern.edu>
wrote:

> Message: 5
> Date: Wed, 27 Jan 2016 14:15:05 +0000
> From: John Shelley <jshelley at sbpdiscovery.org>
> To: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Tissue Arrays
> Message-ID:
>         <C54F513DA7DA7547B37103A4B74BDAE903E4B073 at CARRERA.ln.burnham.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Morning,
>
> I have purchased some arrays from a company in the past and had some very
> varying results. I prefer not to mention the company and so my request is
> if those of you who do not have the luxury of having tissue at your
> disposal to make your own tissue arrays from whom are you buying them from.
> Once purchased have you used them on IHC platforms like Ventana
> Benchmark/Ultra or Leica Bond3? Were your results consistent across the
> slide?
>
> I would like to add that I am looking for neuroblastoma tumor arrays. Any
> help will be greatly appreciated. Thanks in advance!
>
> Kind Regards!
>
> John J Shelley
>