[Histonet] rat vomeronasal organ (VNO) fix, decal, proc. and paraffin embedding protocol

[Margaryan, Naira]

Dear Histonet Colleagues,

I hope you had very nice and warm holiday season .

We are interested in a routinely used protocol to decal (if needed), process and paraffin embed the rat vomeronasal organ (VNO).

It would be greatly appreciated if anyone would be willing to share a protocol for post-harvest sample storage (i.e. PBS, 70% ETOH, saline, etc.), fixation, decalcification, processing before embedding.


Thanks in advance for your help!!

Naira


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