[Histonet] mouse femur decal and paraffin embedding protocol

[Elizabeth Chlipala]

James

We use 10% Formic Acid to decal mouse femur and tibia -  The rear legs are removed at the hip with a pair of rongeurs  the skin is removed to the ankle, we allow the knee joints to fix for 48 hours, they need to be in a normal degree of flexion, meaning you want them in a container that does  not bend the knee.  We trim off the muscle  and place in decal for overnight - process the next day on an hour processing cycle.  You don't need microwave for mouse knees they decal quickly.  Most of the work we do is for cartilage degeneration, rheumatoid or osteoarthritis, if you are looking at bone marrow I would modify slightly.  I can send a work instruction document that has more information in another e-mail if you are interested.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: Herrick, James L. (Jim), MSA via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Monday, January 11, 2016 10:40 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] mouse femur decal and paraffin embedding protocol

Dear Histonet Colleagues,

I hope the holiday season has been good to all.

We are interested in a routinely used protocol to decal, process and paraffin embed mouse femur and tibia. Some of our projects may require a gentle decal, whereas others may not be so delicate. Also, we do have access to a microwave for decalcifying, if recommended to be a better method. It would certainly save a lot of time.

It would be greatly appreciated if anyone would be willing to share a protocol for post-harvest sample storage (i.e. PBS, 70% ETOH, saline, etc.), decalcification, processing and embedding. Thank you in advance for your help!!

Jim Herrick, M.S.A.
Supervisor - Sr. Res.Tech.
Department of Orthopedics
Biomaterials and Histomorphometry Core Lab Biomaterials and Tissue Engineering Lab Office Phone: (507) 538-4300 Lab Phone: (507) 255-5946
Pager: 127 (13239)
Fax: (507) 266-9451
Email: herrick.james at mayo.edu<mailto:herrick.james at mayo.edu>
______________________
Mayo Clinic
200 First Street SW
Rochester, MN 55905
www.mayoclinic.org<http://www.mayoclinic.org/>


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