[Histonet] mouse femur decal and paraffin embedding protocol

[Herrick, James L. (Jim), MSA]

Dear Histonet Colleagues,

I hope the holiday season has been good to all.

We are interested in a routinely used protocol to decal, process and paraffin embed mouse femur and tibia. Some of our projects may require a gentle decal, whereas others may not be so delicate. Also, we do have access to a microwave for decalcifying, if recommended to be a better method. It would certainly save a lot of time.

It would be greatly appreciated if anyone would be willing to share a protocol for post-harvest sample storage (i.e. PBS, 70% ETOH, saline, etc.), decalcification, processing and embedding. Thank you in advance for your help!!

Jim Herrick, M.S.A.
Supervisor - Sr. Res.Tech.
Department of Orthopedics
Biomaterials and Histomorphometry Core Lab
Biomaterials and Tissue Engineering Lab
Office Phone: (507) 538-4300
Lab Phone: (507) 255-5946
Pager: 127 (13239)
Fax: (507) 266-9451
Email: herrick.james at mayo.edu<mailto:herrick.james at mayo.edu>
______________________
Mayo Clinic
200 First Street SW
Rochester, MN 55905
www.mayoclinic.org<http://www.mayoclinic.org/>