[Histonet] H and E Staining in IHC

[Steven Weston]

Hi Marcus,
As stated previously by others there should be no problem putting the Eosin into your sections but I would suggest that you do not leave out the endogenous quenching so you can show your red blood cells. The reason being that there are many other cells that may then show up as positive. Instead I suggest using an Eosin/Phloxine mixture which nicely highlights the red cells differently to the rest of the general matrix material.
regards
Steve Weston
Breathe-Well CRE
Lab Manager
0408990859




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