[Histonet] metal enhanced DAB

[King,Michael A]

Tyrone,
I've had better luck using acetate buffer with metal-enhanced DAB.  There's less of a precipitation problem.  You can also dilute the reaction solution and the reaction product will develop more slowly, or just decrease the peroxide concentration.  You also have some control over the color by adjusting the pH; Co can range from blue to black and Ni from brown to black. 
Good luck,
Mike King

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Wed, 24 Feb 2016 16:08:00 -0600 Tyron Genade wrote:
Subject: [Histonet] metal enhanced DAB
Message-ID:
        <CAEYEE3=dz68k=Gdb=BReKV2MYHaecyx+k07tnth04MdKfwN8=Q at mail.gmail.com>
Hello,
I'm using the Co/Ni enhanced DAB from Histological & Histochemical Methods
(2nd Ed). Does the solution normally turn a blue-gray after adding the
H2O2? I am getting a lot of back ground and precipitate on the sections.
The pH is 7.3.
I had used a similar method for Western Blotting that took 5 minutes for
the solution to change color. This is a near instantaneous color change.
Thanks
Tyrone