[Histonet] metal enhanced DAB

[Tyrone Genade]

Hello,

I'm using the Co/Ni enhanced DAB from Histological & Histochemical Methods
(2nd Ed). Does the solution normally turn a blue-gray after adding the
H2O2? I am getting a lot of back ground and precipitate on the sections.
The pH is 7.3.

I had used a similar method for Western Blotting that took 5 minutes for
the solution to change color. This is a near instantaneous color change.

Thanks

Tyrone